Drug accumulation thickens cell membrane

July 23, 2012

An image gallery of wildtype BY4716 yeast cells treated with 25µM amiodarone for 45 minutes. Amiodarone, a cationic amphipath (fat-soluble weak base), is known to cause phospholipidosis in lab animals chronically treated it.

Here, amiodarone-treated yeast cells accumulated the drug in vacuolar membrane, causing cup formation. Bilayer expansion is evident at the concavities as white stretches in the membrane.

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  • dave_bridges

    How rapidly does the cupping happen? Is this associated with shrinkage or swelling of the vacuole?

    • http://twitter.com/eperlste Ethan Perlstein

      This data is from a 45-minute treatment but we see cupping as soon as 3 minutes. We are writing up a manuscript of a computation analysis of vacuolar properties, including volume/cross-sectional area. Sertraline appears to promote rapid vacuolar fusion, though the total cell volume comprised of vacuoles do not increase compared to untreated controls.